Manal Jamjoom

From Jamjoom

I am Dr. M. B. Jamjoom, Associate professor in the department of Medical Parasitology, Faculty of Medicine at King Abdul Aziz University, Jeddah, Saudi Arabia.

As a teaching academic staff, I participate in teaching the Medical Parasitology course to medical and medical science students. I hope this site will provide you with relevant information about most of the medically important parasites.

In this site, you will find my medical parasitology lecture notes. These cover the various topics that I teach and an atlas library. For those who are interested to know more about me and my research interests, go to my CV.

This site will be frequently revised with updated information related to medical parasitology. If you have any suggestions or concerns regarding the site and its contents, please do not hesitate to contact me. Thank you for your visit.

Contents 1 Research Intersts 2 Education 2.1 Liverpool School of Tropical Medicine (Affiliated to the University of Liverpool, UK) 2.2 King Abdul-Aziz University, Jeddah 2.3 University of Wisconsin at Milwaukee, USA 3 Teaching Experience 4 Work Experience 4.1 Associate professor (2007-present) 4.2 Assistant Professor (2003-2007) 4.3 Coordinator (2003-2007) 4.4 Assistant Supervisor (1992-present) 4.5 Lecturer (1992-2003) 4.6 An Electron-Microscopy Technician (1990-1992) 5 Committees 6 Publications 7 Talks 8 Posters 9 Ph.D. Thesis 10 Masters Thesis 11 Professional Memberships

[edit] Research Intersts

• Cell culture
• DNA isolation
• PCR amplification
• Gene cloning
• DNA sequencing and comparative DNA sequencing
• Electron microscopy
• Water Quality studies
• Assessing the efficiency of new anti-parasitic drugs.
• Parasitic infection & environmental impact.
• Endemic parasitic diseases in Saudi Arabia

[edit] Education

[edit] Liverpool School of Tropical Medicine (Affiliated to the University of Liverpool, UK)

Ph.D., Molecular Protozoa June, 2003

Title: Molecular tools for the classification and identification of Leishmania donovani “complex” Advisors: Professor R. W. Ashford and Dr. P. A. Bates at Liverpool School of Tropical Medicine.

[edit] King Abdul-Aziz University, Jeddah

Masters Degree in Parasitology June, 1990

Thesis: Some Biological Aspects of the Etiological Agent of Cutaneous Leishmaniasis in South western Saudi Arabia Advisors: Prof. A.E. AZ Banaja and Dr. I. M. Shalaby

[edit] University of Wisconsin at Milwaukee, USA

BSc degree in Zoology September, 1984

[edit] Teaching Experience

• Medical parasitology Laboratory material for 3rd year Medical students (1993-1998)
• Medical Parasitology course for 3rd year pharmacology (2003-present)
• Selected topics in Medical Parasitology Course (Cestodes & Tissue protozoa) for 2nd year Nursing (2003-present)
• Selected topics in Medical Parasitology (Leishmaniasis; Malaria & Trypanosomiasis) for 3rd year Dental student (Jan/2003)
• Intestinal Cestodes for 3rd year Medical students (2005)

[edit] Work Experience

[edit] Associate professor (2007-present)

Department of Medical Parasitology King Abdul-Aziz University (Jeddah)

[edit] Assistant Professor (2003-2007)

Department of Medical Parasitology King Abdul-Aziz University (Jeddah)

[edit] Coordinator (2003-2007)

Medical Parasitology Department king Fahd Medical Research Center

[edit] Assistant Supervisor (1992-present)

The Electron Microscopy Unit King Fahd Medical Research Center

[edit] Lecturer (1992-2003)

Medical Parasitology Department, Faculty of Medicine King Abdul-Aziz University (Jeddah)

[edit] An Electron-Microscopy Technician (1990-1992)

The Electron Microscopy Unit King Fahd Medical Research Center

[edit] Committees

• Center for Teaching & Learning Development Committee (2004-2008)
• Medical Educational Unit of Medical Collage Committee (2005-2007)

[edit] Publications

• Shalaby IM, Gherbawy YA and Jamjoom M. Molecular Diagnosis of Cutaneous Leishmaniasis in western region of Saudi Arabia. [Sent for publication].

• Banaja AA, Jamjoom MB, Shalaby IM, Gherbawy YA. Discrimination between susceptible and non-susceptible Biomphalaria alexandrina snails the intermediate hosts of Schistsomiasis in Western Saudi Arabia using random amplified polymorphic DNA analysis. [Sent for publication].

• Jamjoom MB. Contribution of electron microscopic studies to the biology and classification of parasitic cestodes (review article). J Egypt Soc Parasitol. 2007; 37(3 Suppl):1125-1158.

• Jamjoom MB. Review on electron microscopic studies to the taxonomy and biology of parasitic Nemathelminthes. J. Egypt Soc. Parasitol. 2007; 37 (1): 67-105.

• Al-Harthi SA, Jamjoom MB. Enteroparasitic Occurrence in Stools from residents in south-western region of Saudi Arabia before and during Umrah season. Saudi Med. J. 2007; 28 (3): 22-25.

• Jamjoom MB, Banaja AA. Comparative Studies on the Susceptible and Non-Susceptible Biomphalaria alexandrina the Intermediate Snail Host of Schistosoma mansoni in Western Saudi Arabia. W. J. Med. Sci. 2007; 2(2): 108-114.

• Al-Harthi SA, Jamjoom MB. Preliminary study of the prevalence of Intestinal parasites among diarrheic inhabitants in Makkah Al Mukaramah. J. Egypt Soc. Parasitol. 2007; 37(2): 671-680.

• Al-Harthi SA, Jamjoom MB. Diagnosis and Differentiation of Entamoeba Infection in Makkah Al Mukarramah Using Microscopy and Stool Antigen Detection Kits. W. J. Med. Sci. 2007; 2(1): 15-20.

• Jamjoom MB. Shalaby IMI. The contribution of electron microscopic studies to the taxonomy and biology of parasitic trematodes. W. J. Zool. 2006; 1(2): 64-81.

• Jamjoom MB. Molecular identification of some Schistosoma mansoni isolates in Saudi Arabia. W. J. Med. Sci. 2006; 1(2): 102-107.

• Jamjoom MB, Azhar EA, Tonkol AM, Al-Harthi SA, Ashankyty IM. Detection of Malaria in Saudi Arabia by Real-Time PCR. J Egypt Soc Parasitol 2006; 36(3): 737-748.

• Al-Harthi SA, Jamjoom MB, Ghazi HO. Seroprevalence of Toxoplasma gondii infection among pregnant women in Makkah, Saudi Arabia. Umm Al-Qura Univ. J.Sci.Med. Eng. 2006; 18 (2): 217-227.

• Tonkol AM, Salem HS, Jamjoom MB, Altaieb AM, Al-Bar Hassan. Preliminary study on the effect of Ziziphus spina christi, on selected Leishmania species. Sc. J. Az. Med. Fac. (Girls). 2005; 26 (1), 1915-1921.

• Jamjoom MB, Ashford RW, Bates PA, M. Chance, Kemp SI, Noyes HA. L. donovani is the only cause of visceral leishmaniasis in East Africa; previous description of L. infantum and L. archibaldi from this region are a consequence of convergent evolution in isoenzyme data. Parasitology. 2004; 129, 1-11.

• Jamjoom MB, Ashford RW, Bates PA, Kemp SJ, Noyes HA. Polymorphic microsatellite repeats are not conserved between Leishmania donovani and Leishmania major. Mol Ecolo Notes. 2002; 2: 104-106.

• Jamjoom MB, Ashford RW, Bates PA, Kemp SJ, Noyes HA. Towards a standard battery of microsatellite markers for the analysis of the Leishmania donovani complex. Ann Trop Med Parasitol. 2002; 96: 265-270.

• Shalaby IMI, Banaja AA and Jamjoom MB. Ultrastructural observation on the etiological agent of cutaneous Leishmaniasis in South Western Saudi Arabia. Egypt. J. Physiol. Sci. 1996; 21(3): 259-294.

• Shalaby IMI, Banaja AA and Jamjoom MB. Growth rate study on an isolate of the etiological agent of Cutaneous Leishmaniasis in South Western Saudi Arabia grown in three different culture media. Ain Shams Sci. Bull. 1994; 32: 165-173.

• Dehlawi GY, Ismail MF, Hamdi SA, Jamjoom MB. Ultrastructure of spermiogenesis of Saudian reptiles. 6. The sperm head differentiation in Agama adramitana. Arch Androl. 1992; 28(3): 223-234.

[edit] Talks

• Leishmania donovani and L. infantum in the Sudan are a monophyletic group within the L. donovani/L. infantum clade. Presented at the British society for Parasitology BSP meeting: Trypanosomiasis and Leishmaniasis Seminar 8th -11th September 2002, Edinburgh, Scotland.

• Microsatellite Data and chitinase gene sequencing indicate that L. donovani, L. infantum and L. “archibaldi” from Sudan are a polyphyletic assemblage. Presented at the British society for Parasitology BSP meeting: spring meeting 9th- 11th April, 2002, Salford, UK.

• Advances in Malaria Diagnostic & The evaluation of Real-Time PCR for the detection of malaria parasites in the Kingdom of Saudi Arabia. Presented at the 2nd MDG (Molecular Diagnostic Group) meeting 4th-7th December, 2005, Jeddah, SA.

• Comparative Studies on the Susceptible and Non-Susceptible Biomphalaria alexandrina the intermediate snail host of schistosomiasis mansoni in Western Saudi Arabia. Presented at the Third Saudi Science Conference “New Horizons in Science and Their Applications” 10th -13th March 2007.

[edit] Posters

• Microsatellite loci are poorly conserved between Leishmania major and L. donovani. Presented at Genome-based Pathogen Biology: the first 25 years and beyond. 7th -10th July, 2002, Hinxton hall, The Wellcome Trust Genome campus.

• Towards a standard battery of microsatellite markers for the analysis of the Leishmania donovani complex. Presented at the British society for Parasitology BSP meeting: spring meeting 9th- 11th April, 2002, Salford, UK. (This poster was awarded the first prize in the meeting).

• Microsatellite identified in the Leishmania major are not conserved in L. donovani. Presented in WORLDleish2. 20th -24th May, 2001. Creta, Greece.

[edit] Ph.D. Thesis

Summary: Visceral leishmaniasis is a potentially lethal disease. It is clinically presented by viscerotropic dissemination to internal organs. The agents causing the disease are all grouped within the L. donovani “complex” (Lainson and Shaw, 1987). Up to date many issues in the taxonomy within this complex is still controversial; such as the agents causing visceral leishmaniasis in Sudan and the validity and the identity of “L. archibaldi”. The agents causing VL in Sudan are at present defined on the basis of an isoenzyme classification as Leishmania donovani, L. infantum and “L. archibaldi”. “L. archibaldi” only differs from L. donovani in the mobility of one enzyme (GOT). The presence of all three species in Sudan presented was contested by many authors who suggested grouping the zymodemes belonging to these two taxa in a single group L. donovani sensu lato (Ashford et al. 1992).

In this study we have obtained the sequence of the chitinase gene of 37 stocks of these parasites from the Sudan and elsewhere to construct a phylogenetic tree. A panel of microsatellite markers suitable for classifying these species was also developed. Two strategies were used to develop the panel of microsatellites.

Firstly, the Leishmania major genome sequence was used to identify microsatellite markers. Twenty-seven independent microsatellite loci were identified by BLAST search of L. major genome. 13 out of 27 primers designed against the L. major sequences also amplified a single product of approximately the expected size from L. donovani. These 13 microsatellites were tested for variation in a panel of L. donovani “complex”. Only two out of 13 loci that were polymorphic in L. major were also polymorphic in L. donovani. Almost all microsatellite PCR products were significantly smaller in all the test strains than they were in L. major. Therefore, the use of the L. major genome sequence to identify microsatellite loci is unlikely to be an efficient method of identifying microsatellite loci in other Leishmania strains. Secondly, forty microsatellites were identified in L. donovani by an enrichment method (http://WWW.liv.ac.uk/~kempsj/genomics.html.). Primers for twenty of these amplified a single sharp band from L. donovani DNA and were found to be polymorphic between L. donovani stocks. Phylogenetic trees were constructed using microsatellite data.

Both chitinase and microsatellite phylogeny showed that stocks of all three species isolated from Sudan form a single monophyletic group within the Leishmania donovani, / L. infantum clade. We conclude that “L. archibaldi” is not a valid species and the definitions of L. donovani and L. infantum may have to be revised in the light of this data.

[edit] Masters Thesis

Summary: Leishmaniasis is a disease acquired by humans, due to Leishmania parasites, which exist in two forms. A flagellated extracellular promastigotes in the sandfly vector or in vitro culture and aflagellar intracellular amastigotes in the macrophage cells of mammals, lizards and tissue culture.

Leishmaniasis takes several clinical forms including: cutaneous, mucocutaneous and visceral disease. Leishmaniasis and especially cutaneous leishmaniasis was reviewed in terms of epidemiology, biology and isoenzyme electrophoresis. Leishmania research in Saudi Arabia is some what a new undertaking. Cutaneous leishmaniasis is found to be a wide spread disease in Saudi Arabia which eventually affects a large number of people each year and is regarded as a public health problem.

In the present study three human isolates of cutaneous leishmaniasis were obtained from patients living in Abha province, with the objectives of critical investigation on some biological aspects of this organism. These isolates were cultivated in different culture media and the growth rate of promastigotes was assessed quantitatively in three culture media including (NNN, MEM and 199). Animal susceptibility to infection with the three isolates was also tested using syrian hamsters and BALB/c mice. The ultrastructure and morphological characteristics of the parasite were investigated. The three human isolates were also biochemically characterized using isoenzyme electrophoresis technique. The growth rate studies showed that NNN medium was the most suitable culture medium which gave the the highest promastigote count. After inoculating the promastigotes of the three isolates into heal thy Syrian hamsters and BALB/c mice no symptoms of positive infection was observed. Examination of ultra-thin sections of promastigotes of the three isolates using electron microscopy revealed certain consistency of the principal structures of Leishmania promastigotes. Each promastigote possessed an elongated form with a single smooth plasma membrane, a subpellicular layer of microtubules together with many organelles within the cytoplasm. Biochemical characterization of the three isolates was performed by applying the thin layer starch gel electrophoresis technique and using 12 enzyme systems. The activity bands produced by the three isolates were compared with those of four marker strains representing L. major and L. tropica. The three studied isolates were found to represent 2 zymodemes of L. tropica strain.

[edit] Professional Memberships

• Member in the British society for Parasitology (BSP), UK